Simultaneous bioimaging of MMP-2 and MMP-7 via Au-Se constructed fluorescence nanoprobe

• 来源期刊: 中国科学:化学（英文版）
• 年，卷（期）: 2020,（1）
• 页码范围: 135-140
• 页数: 6页
• 语种: 英文

摘要

Au-Se bond strategy has been applied to construct fluorescence nanoprobe that is being used to simultaneously dual-image the tumor markers matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-7 (MMP-7) in vitro.The two Se-terminating ligand peptide chains respectively labeled with fluorescein isothiocyanate (FITC) and 5-Carboxytetramethylrhodamine (5-TAMRA) dyes are attached to the surface ofAu nanoparticles (NPs).The nanoprobe can be specifically cut offby MMP-2 and MMP-7 respectively to carry out significant enhanced fluorescence recovery for simultaneously imaging both markers.The nanoprobe not only displays high selectivity and sensitivity towards MMP-2 and MMP-7,but also has strong anti-interference stability against biothiols and enhanced fidelity for avoiding "false positive" results.This approach offers new perspectives in designing tumor-related early diagnosis approaches and also provides strategies for clinical applications.