Identification of microRNA-mRNA regulatory networks and pathways related to retinoblastoma across human and mouse

• 作者: He Zou Hui Zhang Lu Liu Lu-Fei Wang Mei-Jiao Song Rui Tian
• 来源期刊: 国际眼科杂志:英文版
• 学科: 医学
• 关键词: KYOTO ENCYCLOPEDIA of Genes and GENOMES pathway micro RNA RETINOBLASTOMA weighted gene CO-EXPRESSION network analysis
• 年，卷（期）: 2020,（4）
• 页码范围: 535-544
• 页数: 10页
• 分类号: R73

摘要

AIM: To explore the m RNA and pathways related to retinoblastoma(RB) genesis and development.METHODS: Microarray datasets GSE29683(human) and GSE29685(mouse) were downloaded from NCBI GEO database. Homologous genes between the two species were identified using WGCNA, followed by protein-protein interaction(PPI) network construction and gene enrichment analysis. Disease-related mi RNAs and pathways were retrieved from mi R2 Disease database and Comparative Toxicogenomics Database(CTD), respectively.RESULTS: A total of 352 homologous genes were identified. Two pathways including "cell cycle" and "pathway in cancer" in CTD and enrichment analysis were identified and seven mi RNAs(including hsa-mi R-373, hsa-mi R-34 a, hsami R-129, hsa-mi R-494, hsa-mi R-503, hsa-let-7 and hsami R-518 c) were associated with RB. mi RNAs modulate "cell cycle" and "pathway in cancer" pathways via regulating 13 genes(including CCND1, CDC25 C, E2 F2, CDKN2 D and TGFB2).CONCLUSION: These results suggest that these mi RNAs play crucial roles in RB genesis through "cell cycle" and "pathway in cancer" pathways by regulating their targets including CCND1, CDC25 C, E2 F2 and CDKN2 D.