Cascaded DNA circuits-programmed self-assembly of spherical nucleic acids for high signal amplification

• 来源期刊: 中国科学:化学（英文版）
• 年，卷（期）: 2020,（1）
• 页码范围: 92-98
• 页数: 7页
• 语种: 英文

摘要

Signal amplification is an important issue in DNA nanotechnology and molecular diagnostics.In this work,we report a strategy for the catalytic self-assembly of spherical nucleic acids (SNAs) programmed by two-layer cascaded DNA circuits through integrating an entropy-driven catalytic network,a catalytic hairpin assembly circuit,and a facile SNA assembly-based reporter system.This integrated system could implement ～100,000-fold signal amplification in the presence of 1 pM of input target.Possessing powerful amplification ability of nucleic acid signal,our strategy should be of great potential in fabricating more robust dynamic networks to be applied for signal transduction,DNA computing,and nucleic acid-based diagnostics.