Optimization of the Ethanol Extraction Method of Monoester Alkaloids from Radix Aconiti Preparata Based on HPLC-MS and Orthogonal Test

• 作者: Changkai ZHOU Donghua LIU Fanbo JING Fangang MENG Hongxia YU Hongyan JI Jing GAO Jingchun YAO Lihua ZHOU Na ZHANG Qie GUO Rui ZHANG Xiaomin XING Xiuling SHANG Yongjun LIU Zukang ZHANG
• 来源期刊: 农业生物技术:英文版
• 学科: 化学
• 关键词: Radix aconiti preparata monoester alkaloid HPLC-MS orthogonal test
• 年，卷（期）: 2020,（3）
• 页码范围: 73-77
• 页数: 5页
• 分类号: O62

摘要

[Objectives] This study was conducted to optimize the ethanol extraction technology of monoester alkaloids from Radix Aconiti Preparata. [Methods]On the basis of defined extraction times,ethanol concentration,ethanol times and extraction time were investigated by HPLC-MS combined with orthogonal test to optimize extraction process using the content of monoester alkaloids( the sum of benzoyl neoaconitine,benzoyl hypoaconitine and benzoyl aconitine) as an index.[Results]The optimum ethanol extraction technology was as follows: 75% ethanol,ethanol amount 25 times of the medicinal material,and each extraction for 1. 5 h.[Conclusions] The optimal extraction technology is simple,feasible,stable and reliable. It can provide reference for the industrial production and quality control of monoester alkaloids from Radix Aconiti Preparata.