In situ formation of fluorescent silicon-containing polymer dots for alkaline phosphatase activity detection and immunoassay

• 来源期刊: 中国科学:化学（英文版）
• 年，卷（期）: 2020,（4）
• 页码范围: 554-560
• 页数: 7页
• 语种: 英文

摘要

The discovery and application of analyte-triggered fluorophore generation or fluorogenic reaction are significant and beneficial to the development of novel fluorescence (FL) analysis method.In this study,for the first time,we have reported a fluorogenic reaction to prepare fluorescent silicon-containing polymer dots (Si-PDs) by simply mixing N-[3-(trimethoxysilyl)propyl]ethylenediamine (DAMO) and hydroquinone (HQ) in aqueous solution at ambient temperature.Inspired by the alkaline phosphatase (ALP)-catalyzed hydrolysis of the substrate sodium 4-hydroxyphenyl phosphate (4-HPP) into HQ and the resultant HQ-controlled intense green Si-PDs generation,we have established a straightforward ALP activity assay by innovatively employing commercially available 4-HPP as the substrate.More significantly,the specific preparation method,clear formation mechanism and excellent performance enable the Si-PDs as well as its generation process to develop facile and attractive FL immunoassay.With the help of the universal ALP-based enzyme-linked immunosorbent assay (ELISA) platform and corresponding antibody,a convenient and conceptual ALP-based fluorescent ELISA has been constructed and applied in sensing cardiac troponin I (cTnI),a well-known biomarker of acute myocardial infarction.Our research via in situ formation of fluorescent nanomaterials has great potential application in ALP activity assay,inhibitor screening,and disease diagnosis.