Generation of Virus Free Potato Plantlets through Meristem Culture and Their Field Evaluation

• 作者: Edward Binod Soren Md. Abul Kalam Azad Md. Isrfil Hossen Md. Kamrul Haque Touria El-Jaoual Eaton Zubaida Khatun
• 来源期刊: 美国植物学期刊(英文)
• 学科: 数学
• 关键词: Meristem Culture MICROPROPAGATION Potato Cultivars PLANTLETS Plant Growth Regulators
• 年，卷（期）: 2020,（11）
• 页码范围: 1827-1846
• 页数: 20页
• 分类号: O17

摘要

Different aspects of micropropagation through meristem culture for the production of virus indexed source plants, <i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> tuberization and field evaluation of the </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> regenerated plants were studied on four commercial cultivars of potato (</span><i><span style="font-family:Verdana;">Solanum tuberosum</span></i><span style="font-family:Verdana;"> L.) viz., Diamant, Cardinal, Shilbilati and Lalpakri. The investigation was conducted at Rajshahi, Bangladesh from December 2010 to March 2012 to produce virus-free potato plantlets through meristem culture, shoot multiplications with root induction as well as their acclimatization and evaluation of morphological characters and tuber yield under field condition. Shoot tips of 25</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">-</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">30 day old field</span><span style="font-family:Verdana;">-</span><span style="font-family:;" "=""><span style="font-family:Verdana;">grown plants of above mentioned four cultivars were used for meristem isolation. After isolation, meristems of these varieties of potato were cultured on “M” shaped filter paper bridge in Murashige and Skoog (MS) liquid medium. Four different treatments of media formulations viz. 0.1 mg/L KIN + 0.1 mg/L GA</span>_{<span style="font-family:Verdana;">3</span>}<span style="font-family:Verdana;">, 0.1</span></span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">mg/L KIN + 0.5 mg/L GA</span>_{<span style="font-family:Verdana;">3</span>}<span style="font-family:Verdana;">, 0.5 mg/L KIN + 0.1 mg/L GA</span>_{<span style="font-family:Verdana;">3</span>}<span style="font-family:Verdana;"> and 0.5 mg/L KI</span></span><span style="font-family:;" "=