MicroRNAs (miRNAs) are a class of endogenous small RNAs that regulate target gene expression at the post-transcriptional level.miRNAs play an important role in almost all physiological activities including the processes of embryonic stem cell (ESC) pluripotency maintenance and differentiation, as well as induced pluripotent stem cell (iPSC) reprogramming.In this study, we identified eight novel miRNAs by mining the deep sequencing dataset from mouse ESCs and three kinds of reprogrammed pluripotent cells.Most of them are conserved at least in Murinae animals.Seven of them are derived from gene introns.We further showed that miR-novel-41 is a mirtron that derives from the intron of Man2c1, a protein-coding gene.Mirtrons are a kind of special non-canonical miRNAs encoded in introns.Their precursors are not processed by Drosha but generated by intron splicing.Intron splicing is required for the maturation of mirtrons and allows pre-miRNA-like hairpins to form [1].Both ends of the precursor of a canonical mirtron are precisely generated by the splicing reaction.Some spliced pre-miRNA-like hairpins have a single-stranded tail on either the 5'or 3'end, named as 5'-or 3'-tailed mirtrons, which will be digested by exonuclease to leave a pre-miRNA for Dicer processing [2,3].The potential functions of these novel miRNAs were also preliminarily explored by target genes prediction.