An activatable ratiometric near-infrared fluorescent probe for hydrogen sulfide imaging in vivo

• 作者: Deju Ye Hong-Yuan Chen Liandong Feng Luyan Wu Wenhui Zeng Yidan Sun Yingxiao Yan Yuxuan Hu
• 来源期刊: 中国科学:化学（英文版）
• 年，卷（期）: 2020,（5）
• 页码范围: 741-750
• 页数: 10页
• 语种: 英文

摘要

Ratiometric fluorescent probes hold great promise for in vivo imaging;however,stimuli-activatable ratiometric probes with fluorescence emissions in near-infrared (NIR) region are still very few.Herein,we report a hydrogen sulfide (H2S)-activatable ratiometric NIR fluorescent probe (1-SPN) by integrating a H2S-responsive NIR fluorescent probe 1 into a H2S-inert poly[2,6-(4,4-bis-(2-ethylhexyl)-4H-cyclopenta[2,1-b;3,4-b']dithiophene)-alt-4,7(2,1,3-benzothiadiazole)](PCPDTBT)-based NIR semiconducting polymer nanoparticle (SPN).1-SPN shows "always on" PCPDTBT fluorescence at 830 nm and weak probe 1 fluorescence at 725 nm under excitation at 680 nm.The ratio of NIR fluorescence intensities between 725 and 830 nm (I725/I830) is small.Upon interaction with H2S,the fluorescence at 725 nm is rapidly switched on,resulting in a large enhancement of I725/I830,which is allowed for sensitive visualization and quantification of H2S concentrations in living cells.Taking advantage of enhanced tissue penetration depth of NIR fluorescence,1-SPN is also applied for real-time ratiometric fluorescence imaging of hepatic and tumor H2S in living mice.This study demonstrates that activatable ratiometric NIR fluorescent probes hold great potential for in vivo imaging.