Whole transcriptome analysis on blue light-induced eye damage

• 作者: Bo-Yu Chen Guo-Hui Wang Shao-Jia Guo Xiao-Yun Dong Xin-Li Ouyang Yi-De Wu Yong-Fang Xie
• 来源期刊: 国际眼科杂志:英文版
• 学科: 医学
• 关键词: blue light eye whole transcriptome sequencing gene expression signaling pathways MICE
• 年，卷（期）: 2020,（8）
• 页码范围: 1210-1222
• 页数: 13页
• 分类号: R779.1

摘要

AIM:To analyze abnormal gene expressions of mice eyes exposed to blue light using RNA-seq and analyze the related signaling pathways.METHODS:Kunming mice were divided into an experimental group that was exposed to blue light and a control group that was exposed to natural light.After 14 d,the mice were euthanized and their eyeballs were collected.Whole transcriptome analysis was attempted to analyze the gene expression of the eyeballs using RNA-seq to reconstruct genetic networks.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis were used to reveal the related signaling pathways.RESULTS:The 737 differentially expressed genes were identified,including 430 up and 307 down regulated genes,by calculating the gene FPKM in each sample and conducting differential gene analysis.GO and KEGG pathway enrichment analysis showed that blue light damage may associated with the visual perception,sensory perception of light stimulus,phototransduction,and JAKSTAT signaling pathways.Differential lnc RNA,circ RNA and mi RNA analysis showed that blue light exposure affected pathways for retinal cone cell development and phototransduction,among others.CONCLUSION:Exposure to blue light can cause a certain degree of abnormal gene expression and modulate signaling pathways in the eye.