Network pharmacology-based elucidation of molecular biological mechanisms of Kanglaite injection for treatment of non-small cell lung cancer

• 作者: Bo-Wen Xu Jie Li Jing-Yuan Wu Lu-Chang Cao Shi-Xin Li Wen-Chao Dan Xiao-Xiao Zhang
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2020,（16）
• 页码范围: 48-55
• 页数: 8页
• 语种: 英文

摘要

Objective: To investigate the effective compounds, potential targets and molecular mechanism of Kanglaite injection (KLTi) in the treatment of Non-Small Cell Lung Cancer (NSCLC) based on network pharmacology. Methods: The active compounds and targets of KLTi which extracted and isolated from Coix Seed were screened by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The related genes of NSCLC were obtained by searching the Human Gene Database (GeneCards) and Online Mendelian Inheritance in Man (OMIM). The candidate targets of KLTi in the treatment of NSCLC were obtained after extracting the intersection network. The "drug-component-target-disease" network was constructed with the help of Cytoscape 3.7.2. The Protein- Protein Interaction networks were constructed on the STRING platform and core network modules were screened. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of candidate genes were performed using Metascape platform, and a "pathway-target- compounds" network was constructed to further screen key genes and active compounds. Results: A total of 11 compounds, 22 candidate targets, 206 GO functions and 12 KEGG pathways were obtained. Conclusion: The active compounds of KLTi in the treatment of NSCLC are stigmasterol, stigmasterol α1 and ergosterol. The key targets are PGR, NCOA2, PTGS2, NR3C2, and PTGS1. The core GO functions included receptor activity and binding, neuronal signal transmission and hormone stimulation; KEGG mainly involves cancer pathways, neuroactive ligand-receptor interactions and calcium signaling pathways. This study reveals the molecular biological mechanism of KLTi in the treatment of NSCLC, which is speculated to be related to neuroendocrine, providing a new basis and therapeutic direction for subsequent clinical application and experimental research.

作者信息

序号	姓名	单位	发文数	被引次数	H指数	G指数
1	Bo-Wen Xu		1	0	0.0	0.0
2	Shi-Xin Li		1	0	0.0	0.0
3	Jie Li		1	0	0.0	0.0
4	Xiao-Xiao Zhang		1	0	0.0	0.0
5	Lu-Chang Cao		1	0	0.0	0.0
6	Jing-Yuan Wu		1	0	0.0	0.0
7	Wen-Chao Dan		1	0	0.0	0.0