In vivo combination therapy of chloroquine and stigmasterol against Plasmodium berghei induced pathologies in mice

• 作者: Abdullahi A. Dantani Ahmad M. Kandi Auwal Adamu Buba Hussena Hauwa T. Giaze Mohammed Auwal Ibrahim Morik Dorathy Muhammad A. Saliu Musa Daniel Najib Musa Yaqub Nimat
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2020,（17）
• 页码范围: 10-16
• 页数: 7页
• 语种: 英文

摘要

Objective:To investigate the effect of combination therapy of chloroquine and stigmasterol on Plasmodium (P.) berghei malaria-induced organ pathologies.Methods: Totally 35 mice weighing 20-30g were placed into 7 groups of 5 mice each and distributed as uninfected administered 100 mg/kg BW stigmasterol, uninfected administered only feed and water ad libitum, infected with P. berghei and administered 50 mg/Kg BW stigmasterol, 100 mg/kg BW stigmasterol, 100 mg/kg BW stigmasterol plus 5 mg/kg BW chloroquine, and 5 mg/kg BW chloroquine. The last group of mice served as P. berghei infected and not treated control. The levels of parasitemia, packed cell volume, and other biochemical parameters were measured. Results: Combination therapy of P. berghei infection with stigmasterol and chloroquine did not significantly (P>0.05) reduce parasitemia level while stigmasterol treatment alone significantly (P<0.05) reduced the parasitemia level. However, the P. berghei induced anemia was decreased significantly (P<0.05) upon treatment with a combination of chloroquine and stigmasterol as well as with stigmasterol alone. Furthermore, the combination of chloroquine and stigmasterol significantly (P<0.05) decreased the activities of serum alanine aminotransferase, aspartate aminotransferase, urea and level of spleen total proteins in P. berghei infected mice in comparison with the untreated group. Treatment of P. berghei infected mice with stigmasterol alone and in combination with chloroquine significantly (P<0.05) increased the level of serum creatinine while serum and spleen malondialdehyde levels were significantly (P<0.05) decreased. Levels of glutathione in spleen and kidney were insignificantly (P>0.05) altered upon treatment with both doses of stigmasterol as well as the combination therapy. Conclusions: This study concluded that the combination of stigmasterol and chloroquine could combat anemia and some organ pathologies associated with P. berghei infection.