Preparation of multi-mode probe loaded with cisplatin and Fe3O4 folic acid and its targeting abilityin vitro

• 作者: Da-Yan Yang Lin Xie Ling Lin Min Zhang Ming-Hua Wang Qi-Qing Chen Ting-Ting Zhong Xiang-Xiang Jing Yan Chen
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2020,（17）
• 页码范围: 23-26
• 页数: 4页
• 语种: 英文

摘要

Objective:To prepare a encapsulated liquid fluorocarbon,multimodal nanoscale ultrasonic molecular probes capable of carrying Cisplatin(CDDP)、Fe3O4 and folic acid molecular targeting, study on its basic characteristics and in vitro targeting ability.Methods: The PLGA-Fa、Fe3O4、CDDP and PFP were dissolved in organic solvents in a certain proportion after the folic acid (Fa) molecule which in the surFace of PLGA was attached to polyethylene glycol imide, prepare nanoscale targeted multimodal ultrasound contrast agent PLGA-Fa/Fe3O4/CDDP/PFP,containing Fe3O4、CDDP、PFP by double creaming methods,the basic properties of it,encapsulation efficiency and drug loading of nanometer particle were observed and measured to optimize the optimal dosage of CDDP; the PFFCP and PFCP were acted on in vitro human nasopharyngeal carcinoma (NPC) cell line HNE-1 (high expression of Fa receptor),observe and compare its targeting.Results: Nanoscale targeted multimodal ultrasound contrast agentPLGA-Fa/Fe3O4/CDDP/PFP containing Fe3O4、CDDP、PFP was successfully prepared, the size of the nanometer particle is uniform, the shape is round, the dispersion is good, the diameter distribution is 204nm, and the average electric potential is -15Mv. The nanometer particle could bind to human NPC cell line HNE-1 cells with high expression of Fa receptor, but the nontarget nanometer particle binding of human NPC cell line HNE-1 cells are not obvious.Conclusion: In this study, nanoscale targeted multimodal ultrasound contrast agent PLGA-Fa/Fe3O4/CDDP/PFP containingFe3O4、CDDP、PFP was successfully prepared, and the optimal dose of CDDP was optimized. The nanoparticle have obvious targeting to human NPC cell line HNE-1 cells.