GraS is critical for chloroplast development and affects yield in rice

• 作者: DU Zhi-xuan FU Hai-hui FU Jun-ru HAO Hui-ying HE Hao-hua HE Jin-peng HUANG Zhou WANG Jian-ping XU Jie
• 来源期刊: 农业科学学报（英文）
• 年，卷（期）: 2020,（11）
• 页码范围: 2603-2615
• 页数: 13页
• 语种: 英文
• DOI: 10.1016/S2095-3119(19)62859-5

摘要

Leaf color has been considered an important agronomic trait in rice (Oryza sativa L.) for a long time. The changes in leaf color affect the yield of rice. In this study, a green-revertible albino (graS) mutant was isolated from a 60Co-gamma-irradiated mutant pool of indica cultivar Guangzhan 63-4S. The fine mapping indicated that graS mutant was mapped to chromosome 1, and was located in a confined region between markers ab134 and InDel 8 with genetic distances of 0.11 and 0.06 cM, respectively. Based on the annotation results, four open reading frames (ORFs) were predicted in this region. Sequence analysis revealed that LOC_Os01g55974 had a 2-bp nucleotide insertion (AA) in the coding region that led to premature termination at the 324th base. Sequence analysis and expression analysis of related genes indicated that LOC_Os01g55974 is the candidate gene of GraS. We studied the genome and protein sequences of LOC_Os01g55974, and the data showed that GraS contains a deoxycytidine deaminase domain, which was expressed ubiquitously in all tissues. Further investigation indicated that GraS plays an essential role in the regulation of chloroplast biosynthesis, photosynthetic capacity and yield.