Deformability cytometry for high-throughput cell mechanical phenotyping

• 来源期刊: 科学通报（英文版）
• 年，卷（期）: 2020,（24）
• 所属期刊栏目: RESEARCH HIGHLIGHTS
• 页码范围: 2045-2047
• 页数: 3页
• 语种: 英文

摘要

Flow cytometry has become a powerful cell analysis technique for modern clinical diagnosis and biological research.Even though great success has been achieved using flow cytometry,this tech-nique still suffers from some limitations,such as the use of specific immune biomarkers and fluorescent dyes.In contrast,the novel deformability cytometry achieves label-free identification of cells according to their different mechanical properties.The cell mechanical property is an inherent biophysical marker that indi-cates the cell physiological state or function associated with vari-ous diseases[1].Standard approaches for assessing cell deformability include atomic force microscopy,micropipette aspi-ration,microbead rheometry,and optical tweezers[2].However,these approaches have extremely low throughput(typically less than 1 cell/min),are technically demanding,and require labor-intensive operations.