One-step polymerized lanthanide-based polystyrene microsphere for sensitive lateral flow immunoassay

• 作者: Zhenhua Li Qingyun Liu Yongfang Li Wei Yuan Fuyou Y.Li
• 来源期刊: 稀土学报（英文版）
• 年，卷（期）: 2021,（1）
• 所属期刊栏目: SPECTROSCOPY,LUMINESCENCE AND PHOSPHORS
• 页码范围: 11-18
• 页数: 8页
• 语种: 英文

摘要

Lateral flow immunoassays (LFIAs) provide a powerful tool for rapid real-time assay of cancer biomarkers,which is vital for cancer detection and treatment follow-up.Lanthanide-based LFIAs is one of the most widely used methods,especially Eu (Ⅲ) chelates,which possess distinctive and attractive characteristics,such as time-resolved fluorescence and large Stokes shift.Herein,we adopted a new onestep mini-emulsion polymerization method to synthesize carboxyl-modifled fluorescent microsphere (OS-EuCM),which shows good stability,resistance to non-specific adhesion and uniform particle size distribution compared with traditional microspheres synthesized through the swelling method.Benefiting from the above advantages,OS-EuCM was successfully used in LFIAs to detect tumor marker Alpha-fetoprotein with high sensitivity and selectivity in concentration as high as 320 ng/mL,as well as a detection limit of 0.683 ng/mL.This lanthanide-based microsphere holds great potential for rapid point-of-care screening and clinical application.