Batch CGTase Production with Free and Immobilized <i>Bacillus firmus</i>Strain 37 in Bovine Bone Charcoal

• 作者: Larissa Albunio Silva Graciette Matioli Gisella Maria Zanin Flavio Faria Moraes
• 来源期刊: 化学工程与科学期刊(英文)
• 学科: 经济
• 关键词: Bacillus firmus CGTASE Bone Charcoal Matrix Fluidized Bed Batch Reactor Cell Immobilization
• 年，卷（期）: 2021,（1）
• 页码范围: 91-104
• 页数: 14页
• 分类号: F42

摘要

The present study aimed to study the batch production of CGTase (cyclomaltodextrin-</span><span style="font-family:Verdana;">glucanotransferase</span><span style="font-family:Verdana;">) with </span><i><span style="font-family:Verdana;">Bacillus </span><span style="font-family:Verdana;">firmus</span></i><span style="font-family:Verdana;"> strain 37 free and immobilized in bovine bone charcoal in batch mode and </span><span style="font-family:Verdana;">in</span><span style="font-family:Verdana;"> a fluidized bed batch reactor, respectively. The bovine bone charcoal is an innovative support material for the immobilization of microorganisms’ producers of enzymes and the use of this microbial support allows its reuse to a significant cost reduction of the process. The batch fermentation with free cells was investigated for 96 h and reached a CGTase activity equal to 0.77 U/mL. When the microorganism was immobilized on bovine bone charcoal (7 g) and cultivated in </span><span style="font-family:Verdana;">fluidized</span><span style="font-family:Verdana;"> bed batch reactor with air supplementation (1 volume of air/volume of medium * minute), the same activity could be achieved in 24 h. The results of enzymatic activity achieved </span><span style="font-family:Verdana;">show</span><span style="font-family:Verdana;"> the potential of CGTase production in a short time with </span><i><span style="font-family:Verdana;">Bacillus </span><span style="font-family:Verdana;">firmus</span></i><span style="font-family:Verdana;"> strain 37 immobilized in </span><span style="font-family:Verdana;">bovine</span><span style="font-family:Verdana;"> bone charcoal matrix and using air supplementation in the production medium.