Gene deficiency or pharmacological inhibition of PDCD4-mediated FGR signaling protects against acute kidney injury

• 作者: Xu Jing Dandan Ren Fei Gao Ye Chen Xiao Wu Yue Han Qingsheng Han Liang Li Xiaojie Wang Wei Tang Yan Zhang
• 来源期刊: 药学学报（英文版）
• 年，卷（期）: 2021,（2）
• 所属期刊栏目: Original articles
• 页码范围: 394-405
• 页数: 12页
• 语种: 英文

摘要

Recent studies have shown that programmed cell death 4(PDCD4)modulates distinct signal transduction pathways in different pathological conditions.Despite acute and chronic immune responses elicited by ischemia contributing to the functional deterioration of the kidney,the contributions and mechanisms of PDCD4 in acute kidney injury(AKI)have remained unclear.Using two murine AKI models including renal ischemia/reperfusion injury(IRI)and cisplatin-induced AKI,we found that PDCD4 deficiency markedly ameliorated renal dysfunction and inflammatory responses in AKI mice.Consistently,upregulation of PDCD4 was also confirmed in the kidneys from patients with biopsy confirmed acute tubular necrosis from a retrospective cohort study.Moreover,we found that overexpres-sion of Fgr,a member of the tyrosine kinase family,dramatically aggravated renal injury and counter-acted the protective effects of PDCD4 deficiency in AKI mice.We discovered that FGR upregulated NOTCH1 expression through activating STAT3.Most importantly,we further found that systemic admin-istration of ponatinib,a tyrosine kinase inhibitor,significantly ameliorated AKI in mice.In summary,we identified that PDCD4 served as an important regulator,at least in part,of FGR/NOTCH1-mediated tubular apoptosis and inflammation in AKI mice.Furthermore,our findings suggest that ponatinib-