Efficacy of Sanqi(Radix Notoginseng)in treating cerebral hemor-rhage in rats with traumatic brain injury

• 作者: JIANG Tao ZHOU Xiaqing JIANG Huifang YING Rongbiao ZHANG Zhirong CAI Danli WU Yu FANG Hai-jun WANG Lingcong
• 来源期刊: 中医杂志（英文版）
• 年，卷（期）: 2021,（2）
• 所属期刊栏目: Research Articles
• 页码范围: 262-269
• 页数: 8页
• 语种: 英文
• DOI: 10.19852/j.cnki.jtcm.2021.02.006

摘要

OBJECTIVE:To evaluate the protective efficacy of Sanqi(Radix Notoginseng)on cerebral hemorrhage in a rat model of traumatic brain injury(TBI)by in-vestigating plasminogen activator inhibitor-1(PAI-1),tissue-type plasminogen activator(t-PA),nuclear factor-KB(NF-κB,p-p65),nitric oxide(NO),endothelin(ET),cluster differentiation(CD61CD62),and coagulation.METHODS:The free-fall method was used to cre-ate a rat model of TBI.Forty-eight rats were ran-domly divided into six groups:the blank group,sham group,model group,low-dose Sanqi(Radix Notoginseng)group,middle-dose Sanqi(Radix No-toginseng)group,and high-dose Sanqi(Radix No-toginseng)group.At 24 h after the model was creat-ed,we investigated brain MRI,brain tissue morphol-ogy using HE staining,flow cytometry,and immu-nohistochemical changes.RESULTS:Cerebral hemorrhage was aggravated in TBI rats(observed in brain specimens,brain MRI,and brain tissue HE).Cerebral immunohistochemis-try results demonstrated that the expression of t-PA,PAI-1 and p-p65 increased significantly in TBI rats,while t-PA/PAI-1 had a significant decrease.In addition,CD61CD62,D2D,and ET were significant-ly increased in TBI rats,and PT and APTT were sig-nificantly prolonged;in contrast,NO was signifi-cantly decreased.Sanqi(Radix Notoginseng)de-creased cerebral hemorrhage in TBI rats(observed in brain MRI and brain tissue HE),and increased t-PA/PAI-1,CD61CD62 significantly.It also signifi-cantly decreased the expression of t-PA,PAI-1,and p-p65 in brain immunohistochemistry and signifi-cantly decreased PT,APTT,D2D,and ET.However,there were no differences in NO between the mod-el group and the Sanqi(Radix Notoginseng)group.CONCLUSION:Sanqi(Radix Notoginseng)can de-crease the expression of p-p65,increase t-PA/PAI-1,and stem traumatic intracranial hemorrhage in a TBI rat model.