Antifungal Mechanism of the Combination of Eugenol with Ketoconazole Against Malassezia

• 作者: LIU Di HAN Lin GUO Qing-quan
• 来源期刊: 中国生物医学工程学报（英文版）
• 学科: 工学
• 年，卷（期）: 2021,（3）
• 所属期刊栏目: Research papers
• 页码范围: 93-104
• 页数: 12页
• 分类号: TQ658.3
• 语种: 英文

摘要

Malassezia species are common pathogenic fungus that causes several skin diseases, which have a certain rate of resistance to antifungal drugs. Attention has been brought to aromatic compounds because of their promising antimicrobial properties. The present study aims to investigate the mechanism of the anti-Malassezia activity of eugenol and to evaluate its' potential synergism with ketoconazole. The minimum inhibitory concentration (MIC) of eugenol and ketoconazole were investigated using a standard microbroth dilution assay. It was found that eugenol and ketoconazole have a synergistic effect with the FICI value 0.5 through the checkerboard assay. The effects of eugenol and its' combination with ketoconazole on the cell structure (SEM, TEM), the cell wall (sorbitol protection effect), and the cell membrane (the release of intracellular material, complex with the ergosterol, ergosterol synthesis) were investigated. The results of these studies on the mechanisms of action suggested that eugenol exerts antifungal effects on the cell wall and cell membrane of M. furfur, the biosynthesis of ergosterol was also being disturbed. The destruction was strengthened when combined with ketoconazole.