Divergent Projection Patterns Revealed by Reconstruction of Individual Neurons in Orbitofrontal Cortex

• 作者: Junjun Wang Pei Sun Xiaohua Lv Sen Jin Anan Li Jianxia Kuang Ning Li Yadong Gang Rui Guo Shaoqun Zeng Fuqiang Xu Yu-Hui Zhang
• 来源期刊: 神经科学通报（英文版）
• 年，卷（期）: 2021,（4）
• 所属期刊栏目: Original Articles
• 页码范围: 461-477
• 页数: 17页
• 语种: 英文

摘要

The orbitofrontal cortex (OFC) is involved in diverse brain functions via its extensive projections to multiple target regions.There is a growing understanding of the overall outputs of the OFC at the population level,but reports of the projection patterns of individual OFC neurons across different cortical layers remain rare.Here,by combining neuronal sparse and bright labeling with a whole-brain florescence imaging system (fMOST),we obtained an uninterrupted three-dimensional whole-brain dataset and achieved the full morphological reconstruction of 25 OFC pyramidal neurons.We compared the wholebrain projection targets of these individual OFC neurons in different cortical layers as well as in the same cortical layer.We found cortical layer-dependent projections characterized by divergent patterns for information delivery.Our study not only provides a structural basis for understanding the principles of laminar organizations in the OFC,but also provides clues for future functional and behavioral studies on OFC pyramidal neurons.