Novel alternative splicing of GABA receptor RDL exon 9 from Laodelphax striatellus modulates agonist potency

• 作者: Jie Jiang Li-Xin Huang Feng Chen Cheng-Wang Sheng Qiu-Tang Huang Zhao-Jun Han Chun-Qing Zhao
• 来源期刊: 中国昆虫科学（英文版）
• 年，卷（期）: 2021,（3）
• 所属期刊栏目: Original articles
• 页码范围: 757-768
• 页数: 12页
• 语种: 英文
• DOI: 10.1111/1744-7917.12789

摘要

The resistance to dieldrin gene(RDL)encodes the primary subunit of the insect ionotropic γ-aminobutyric acid(GABA)receptor(GABAR),which is the target of phenylpyrazole and isoxazoline insecticides.The splice variants in exons 3 and 6 of RDL,which have been widely explored in many insects,modulate the agonist potency of the homomeric RDL GABAR and potentially play an important role in the development of insects.In the present study,four splice variants of exon 9 were identified in RDL of the small brown planthopper,Laodelphax striatellus(LsRDL),resulting in LsRDL-9a,LsRDL-9a',LsRDL-9b,and LsRDL-9c.LsRDL-9a has one more amino acid(E,glutamic acid)compared with LsRDL-9a',and LsRDL-9b lacked two amino acids and had seven different amino acids compared with LsRDL-9c.Two-electrode voltage-clamp recording on LsRDLs expressed in Xenopus oocytes showed that alternative splicing of exon 9 has significant impact on LsRDL sensitivity to the agonists GABA and β-alanine,whereas no significant difference was observed in the potencies of the non-competitive antagonists(NCAs)ethiprole and fluralaner on the splice variants.Our results suggest that alternative splicing of RDL exon 9 broadens functional capabilities of the GABAR in L.striatellus by influencing the action of GABA.