Distinct Characteristics of Odor-evoked Calcium and Electro-physiological Signals in Mitral/Tufted Cells in the Mouse Olfactory Bulb

• 作者: Han Xu Chi Geng Xinzhong Hua Penglai Liu Jinshan Xu Anan Li
• 来源期刊: 神经科学通报（英文版）
• 年，卷（期）: 2021,（7）
• 所属期刊栏目: Original Articles
• 页码范围: 959-972
• 页数: 14页
• 语种: 英文

摘要

Fiber photometry is a recently-developed method that indirectly measures neural activity by moni-toring Ca2+signals in genetically-identified neuronal populations.Although fiber photometry is widely used in neuroscience research,the relationship between the recorded Ca2+signals and direct electrophysiological measurements of neural activity remains elusive.Here,we simultaneously recorded odor-evoked Ca2+and elec-trophysiological signals[single-unit spikes and local field potentials(LFPs)]from mitral/tufted cells in the olfactory bulb of awake,head-fixed mice.Odors evoked responses in all types of signal but the response characteristics(e.g.,type of response and time course)differed.The Ca2+signal was correlated most closely with power in the 3-band of the LFP.The Ca2+signal performed slightly better at odor classification than high-γ oscillations,worse than single-unit spikes,and similarly to β oscillations.These results provide new information to help researchers select an appropriate method for monitoring neural activity under specific conditions.