Immunomodulatory effects of astragalus polysaccharide on Human peripheral blood mononuclear cells co-cultured with cervical cancer cell line

• 作者: Elham Shokati Mohammad-Reza Shokri Kobra Entezami Samaneh Khorrami Mona Amani Morteza Motallebnezhad Elahe Safari
• 来源期刊: 中医杂志（英文版）
• 年，卷（期）: 2021,（5）
• 所属期刊栏目: Research Articles
• 页码范围: 684-694
• 页数: 11页
• 语种: 英文
• DOI: 10.19852/j.cnki.jtcm.2021.05.004

摘要

OBJECTIVE:To investigate immunomodulatory ef-fects of Astragalus polysaccharides (APS) on the co-culture of peripheral blood mononuclear cells(PBMCs) with HeLa cervical cancer cell line.METHODS:To assess the proliferation of PBMCs,carboxyfluorescein succinimidyl ester (CFSE)-Ia-beled PBMCs were co-cultured with HeLa cells and treated with different concentrations of APS.Super-natants of cell culture were collected for cytokines assay via enzyme-linked immunosorbent assay (ELI-SA).The impact of APS on the proliferation of PBMCs,induction of regulatory T cells (Tregs),and myeloid-derived suppressor cells (MDSCs) was car-ried out by flow cytometry.RESULTS:It was observed that APS could increase the proliferation of PBMCs co-cultured with HeLa cells (P ＜ 0.05).However,APS had no significant ef-fects on the induction of Tregs and MDSCs in the co-culture assay (P ＞ 0.05).Furthermore,ELISA re-sults demonstrated that APS could decrease IL-10 and TGF-β3 concentration (P ＜ 0.05).CONCLUSION:The above-mentioned characteris-tics showed that APS might be able to modulate immune responses and improve anti-tumor effects through increasing the proliferation of PBMCs and decreasing inhibitory cytokines secretion as critical mediators of immune suppression in the tumor mi-croenvironment.