The Nitrogen-Cycling Network of Bacterial Symbionts in the Sponge Spheciospongia vesparium

• 作者: HE Liming KARLEP Liisi LI Zhiyong
• 来源期刊: 中国海洋大学学报（自然科学英文版）
• 年，卷（期）: 2021,（4）
• 所属期刊栏目: Life and Fisheries Sciences
• 页码范围: 999-1012
• 页数: 14页
• 语种: 英文

摘要

The microbes associated with sponges play important roles in the nitrogen cycle of the coral reefs ecosystem,e.g.,nitri-fication,denitrification,and nitrogen fixation.However,the whole nitrogen-cycling network has remained incomplete in any indi-vidual sponge holobiont.In this study,454 pyrosequencing of the 16S rRNA genes revealed that the sponge Spheciospongia ve-sparium from the South China Sea has a unique bacterial community (including 12 bacterial phyla),dominated particularly by the genus Shewanella (order Alteromonadales).A total of 10 functional genes,nifH,amoA,narG,napA,nirK,norB,nosZ,ureC,nrfA,and gltB,were detected in the microbiome of the sponge S.vesparium by gene-targeted analysis,revealing an almost complete nitro-gen-cycling network in this sponge.Particularly,bacterial urea utilization and the whole denitrification pathway were highlighted.MEGAN analysis suggests that Proteobacteria (e.g.,Shewanella) and Bacteroidetes (e.g.,Bizionia) are probably involved in the ni-trogen cycle in the sponge S.vesparium.