Effect of inhibiting miR-155 expression on proliferation and migration of airway smooth muscle cell in patients with COPD

• 作者: Wei Wang Si-Lin Zhao Fu-Yuan Fan Zhao-Hui Jin Da Li Yan Fu Shuang Sun Xue-Fei Xiao Hui-Qing Zhang Li Hu
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2021,（10）
• 所属期刊栏目: Original article
• 页码范围: 41-45
• 页数: 5页
• 语种: 英文

摘要

Objective: To investigate the effect of inhibiting miR-155 expression on the proliferation and migration of airway smooth muscle cells (ASMCs) in patients with chronic obstructive pulmonary disease (COPD). Methods: ASMCs were isolated and cultured from 8 patients with COPD (observation group) and 3 patients with benign lung cancer without COPD (control group). The ASMCs were transfected with miR-155 suppression expression plasmid ( to detect the expression of miR-155; flow cytometry was used to detect the cell cycler of cell clones; Transwell was used to detect cell migration and invasion; Enzyme-linked immunosorbent aanti-miR-155) and the negative contre; clone formation experiment was used to detect the numbol plasmid (anti-miR-NC), and blank control group was set. Real-time quantitative PCR (RT-qPCR) was usedssay (ELISA) method was used to detect tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) level. Results: The expression level of miR-155 in ASMCs of observation group was significantly higher than that in the control group (P<0.05). The miR-155 expression level in inhibited miR-155 expression group was significantly lower,compared with the negative control group and the blank group (P<0.05). In the inhibited miR-155 expression group, the proportion of G0-G1 phase cells was increased, the proportion of S phase cells was decreased, the number of cell clones, migration, and the number of invasive cells were decreased, and the levels of TNF-α and IL-6 were increased (P<0.05). Conclusions: Inhibiting the expression of miR-155 can inhibit the proliferation and migration of airway smooth muscle cells in COPD patients, and inhibit the release of proinflammatory factors.