CRISPR/Sc++-mediated genome editing in rice

• 作者: Guigen Ma Yongjie Kuang Zhenwan Lu Xueqi Li Ziyan Xu Bin Ren Xueping Zhou Huanbin Zhou
• 来源期刊: 植物学报（英文版）
• 年，卷（期）: 2021,（9）
• 所属期刊栏目: Breakthrough Reports
• 页码范围: 1606-1610
• 页数: 5页
• 语种: 英文

摘要

Streptococcus canis Cas9 (ScCas9) is an RNA-guided endonuclease with NNG protospacer adjacent motif (PAM) specificity whose genome-editing activity in rice is locus-dependent. Here we investigated the performance of a ScCas9 variant named Sc++ at different NNG PAM sites in the rice genome; Sc++ harbors a T1227K mutation and the substitution of a positively charged loop (residues 367–376). Sc++nuclease achieved broader genome editing compared to the original ScCas9, and its nickase improved targeted base editing in transgenic rice plants. Using the high-efficiency adenine base editor rBE73b, we generated many new OsGS1 alleles suitable for screening of rice germplasm for potential herbicide resistance in the future. The CRISPR/Sc++ system expands the genome-editing toolkit for rice.