Identification of noncoding RNA-encoded proteins on lipid droplets

• 作者: Ting Huang Adekunle T.Bamigbade Shimeng Xu Yaqin Deng Kang Xie Ololade O.Ogunsade Ahmed Hammad Mirza Jifeng Wang Pingsheng Liu Shuyan Zhang
• 来源期刊: 科学通报（英文版）
• 年，卷（期）: 2021,（4）
• 所属期刊栏目: SHORT COMMUNICATIONS
• 页码范围: 314-318
• 页数: 5页
• 语种: 英文

摘要

With the rapid development of high-throughput sequencing technologies, it has recently become evident that the eukaryotic genome is extensively transcribed [1,2].Large-scale projects for the systematic annotation and functional characterization of genes,such as GENCODE and FANTOM, have demonstrated that at least 80％ of mammalian genomic DNA is actively transcribed.It has become increasingly clear that many genomic regions classified as noncoding actually harbor short open reading frames (sORFs) with fewer than 300 nucleotides, encoding small, functional proteins or micropeptides.These small proteins have evaded detection due to their small size, low abundance, instability, and loss during sample preparation [3].However, emerging experimental techniques, including ribosome profiling and mass spectrometry, along with computational advances have enabled their discovery and analysis [3,4].Most have been identified in prokaryotic organisms [4], but a growing number have also been discovered in eukaryotic organisms, including plants, zebrafish, insects, mice, and humans [2-4].These proteins have been found to play important roles in many fundamental biological processes [3] and have been reported to localize to cellular compartments such as mitochondria [5], late endosomes/lysosomes [6], and endoplasmic reticulum [7].However, given the large number of putative proteins encoded by noncoding RNAs, there is a need for much more investigation.In particular, there is a need for more information on the subcellular localization of these proteins, which may provide insight into their biological functions.