Iron-Imprinted Single-Atomic Site Catalyst-Based Nanoprobe for Detection of Hydrogen Peroxide in Living Cells

• 作者: Zhaoyuan Lyu Shichao Ding Maoyu Wang Xiaoqing Pan Zhenxing Feng Hangyu Tian Chengzhou Zhu Dan Du Yuehe Lin
• 来源期刊: 纳微快报（英文版）
• 年，卷（期）: 2021,（9）
• 页码范围: 494-506
• 页数: 13页
• 语种: 英文

摘要

Fe-based single-atomic site catalysts (SASCs),with the natural metalloproteases-like active site structure,have attracted widespread attention in biocatalysis and biosensing.Precisely,control-ling the isolated single-atom Fe-N-C active site structure is crucial to improve the SASCs' performance.In this work,we use a facile ion-imprinting method (IIM) to synthesize isolated Fe-N-C single-atomic site catalysts (IIM-Fe-SASC).With this method,the ion-imprinting process can precisely control ion at the atomic level and form numerous well-defined single-atomic Fe-N-C sites.The IIM-Fe-SASC shows bet-ter peroxidase-like activities than that of non-imprinted references.Due to its excellent properties,IIM-Fe-SASC is an ideal nanoprobe used in the colorimetric biosensing of hydrogen peroxide (H2O2).Using IIM-Fe-SASC as the nanoprobe,in situ detection of H2O2 generated from MDA-MB-231 cells has been successfully demonstrated with satisfactory sensitivity and specificity.This work opens a novel and easy route in designing advanced SASC and provides a sensitive tool for intracellular H2O2 detection.