On-chip circulating tumor cells isolation based on membrane filtration and immuno-magnetic bead clump capture

• 作者: Shuai Zhang Yue Wang Chaoqiang Yang Junwen Zhu Xiongying Ye Wenhui Wang
• 来源期刊: 纳米技术与精密工程（英文版）
• 年，卷（期）: 2022,（1）
• 所属期刊栏目: RESEARCH ARTICLES
• 页码范围: 24-34
• 页数: 11页
• 语种: 英文

摘要

Isolating rare circulating tumor cells(CTCs)from blood is critical for the downstream analysis that is important in cancer-related research,diagnosis,and medicine,and efforts are ongoing to increase the efficiency and purity of CTC isolation in microfluidics.Reported in this paper is a two-stage integrated microfluidic chip for coarse-to-fine CTC isolation from whole blood.First,blood cells are removed by filtration using a micropore-array membrane,then CTCs and other cells that are trapped in the micropores are peeled off the membrane by a novel release method based on air-liquid interfacial tension,which significantly increases the recovery rate of CTCs.The second stage is CTC capture based on an on-chip dense immuno-magnetic-bead clump,which offers high capture efficiency and purity.Both the micropore filtration and immuno-magnetic-bead capture are validated and optimized experimentally.Overall,the integrated microfluidic chip can realize a recovery rate of 85.5％and a purity of 37.8％for rare cancer cells spiked in whole blood.