Mechanism of calcium hydroxide inhibiting methicillin resistant Staphylococcus aureus

• 作者: Zheng-Dong Guo Yang-Yang Bian Xiao-Qian Liu Dong Wang Si-Yuan Zhang Jian Yang Lei Peng
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2022,（5）
• 所属期刊栏目: Original article
• 页码范围: 18-22
• 页数: 5页
• 语种: 英文

摘要

Objective:To investigate the inhibitory effect of calcium hydroxide on methicillin-resistant Staphylococcus aureus and the related inhibition mechanism. Methods: To determine the minimum inhibitory concentration of calcium hydroxide using microplate dilution method; to compare the effects of calcium hydroxide at 8MIC, MIC, 1/4MIC and 0 concentrations on MRSA using growth curve method; to determine the effects of calcium hydroxide on the cell membrane of methicillin-resistant Staphylococcus aureus using calcium xanthophyll and propidium iodide fluorescence staining The effect of calcium hydroxide on the morphology of methicillin-resistant Staphylococcus aureus was observed by scanning electron microscopy; the inhibition mechanism of calcium hydroxide on MRSA was investigated by sodium dodecyl sulfate polyacrylamide gel electrophoresis of protein bands. Results: The MIC of calcium hydroxide on MRSA was 3.125 mg/mL; the fluorescence intensity showed significant changes after co-culture of calcium hydroxide with bacteria; MRSA appeared to be significantly crumpled and broken in the presence of calcium hydroxide; the SDS-PAGE experimental bands indicated that the protein inside the bacteria decreased accordingly with the increase of calcium hydroxide concentration. Conclusion: Calcium hydroxide has a significant inhibitory effect on the growth of MRSA, and its bactericidal mechanism may be related to the destruction of bacterial body structure.