The stability of variable period sampling control systems is studied by using Lyapunov-like functional. In the process of constructing Lyapunov-like functional, the dimension of a matrix in the functional is increased to make full use of the sampling information of the system. In the process of estimating Lyapunov-like functional derivatives, the existing relevant integral inequalities are fully used for scaling and shrinking. Finally, a less conservative system stability criterion is derived by using linear matrix inequality technique. Numerical examples show that the proposed system stability theorem is less conservative, the effectiveness and the superiority of the method are verified.