CONSTRUCTION AND IN VITRO EXPRESSION OF STREPTOCOCCUS MUTANS SURFACE PROTEIN ENCODING DNA VACCINE

• 来源期刊: 同济医科大学学报(外文版)
• 学科: 医学
• 关键词: 结构基因克隆 口腔医学 明文 数据分析 生物学 变形链球菌 表面蛋白
• 年，卷（期）: 2001,（4）
• 页码范围: 269-272
• 页数: 4页
• 分类号: R392
• 语种: 中文

摘要

DNA vaccine plasmids were constructed that encoded two highly conservative regions of a surface protein, PAc, from the human major cariogenic bacterium, Streptococcus mutans . Antigen expression was evaluated in vitro by immunohistochemical analysis of human endothelial cells following cationic liposome mediated transient transfection with recombinant plasmid. The results of this study provided a basis for further testing of these recombinant plasmids in primates and for efficacy testing of dental caries DNA vaccines in human volunteers in future.