rho家族的小分子量G蛋白介导缓激肽引起的猪冠状动脉松弛

• 作者: Paul M VANHOUTTE Toshiro SHIBANO
• 来源期刊: 中国药理学报（英文版）
• 学科: 医学
• 关键词: 缓激肽 内皮 G蛋白 一氧化氮
• 年，卷（期）: 2003,（11）
• 所属期刊栏目: 研究原著
• 页码范围: 1070-1076
• 页数: 7页
• 分类号: R318
• 语种: 英文

摘要

AIM: To determine whether or not low molecular G-proteins are involved in the endothelium-dependent relaxations to bradykinin. METHODS: The effects of botulinum ADP-ribosyltranferase C3 were studied in porcine coronary arteries and endothelial cells. RESULTS: Incubation of membrane fractions isolated from endothelial cells with the enzyme and 32p-NAD resulted in the ribosylation of the proteins with molecular weight of 24-25 kDa. Radio labelling of these proteins was suppressed in the presence of guanosine 5t-O-(3-thiotriphosphate) (GTP-yS), a hydrolysis-resistant analog of GTP. In the isolated arteries, ADP-ribosyltransferase C3 attenuated the relaxations tobradykinin during contractions with prostaglandin F2α in the presence of tween 80 (non ionic detergent), but not in the absence of tween 80. CONCLUSION: Low molecular weight G-proteins of the Rho family contribute to the mechanism of relaxation induced by bradykinin.