Helicobacter pylori infection is associated with increased expression of macrophage migration inhibitory factor by T cells and macrophages in gastric mucosa

• 作者: Harry Hua Xiang XIA HE Xing Xiang LIN Man Peng LIU Wei SHEN Qing Yan ZHAO Ying Heng ZHENG Xue Ling
• 来源期刊: 中华腹部疾病杂志
• 学科: 医学
• 关键词: Macrophage migration inhibitory factor Helicobacter pyloriL inflammation T cells macrophages
• 年，卷（期）: 2004,（9）
• 所属期刊栏目: 论著
• 页码范围: 625-634
• 页数: 10页
• 分类号: R573.6
• 语种: 中文

摘要

AIM Macrophage migration inhibitory factor (MIF) plays a pivotal role in inflammatory/immune diseases.This study aimed to determine MIF expression in H.pylori-induced gastritis,and the effect of H.pylori on MIF expression in monocytes in vitro.METHODS Seventy-nine patients (M/F,39/40,mean age,52 yrs) referred for upper endoscopy were selected;19 with gastric ulcer,15 with duodenal ulcer and 45 with non-ulcer dyspepsia (NUD).Gastric antral and body biopsies were obtained for histological examinations,double immunostaining for MIF/T-cells (CD45RO) and MIF/macrophage (KP1),and in situ hybridization for the expression of MIF mRNA.THp-1,a monocyte cell line,was co-incubated with different concentrations of the whole cell proteins prepared from H.pylori strain ATCC26695 or its isogenic type with cagA gene deleted.The expression of MIF protein was determined by using enzyme linked immunosorbent assay and the MIF mRNA by retrospective transcription-polymerase chain reaction techniques.RESULTS H.pylori was detected in 50 patients (10 with gastric ulcer, 15 with duodenal ulcer and 25 with NUD).Overall,the numbers of total T-cells,MIF+T-cells,total macrophages,MIF+macrophages and MIF mRNA+ cells were greater in the gastric antrum than in the body.There was a significant increase in the numbers of total T-cells, MIF+ T-cells,total macrophages,MIF+macrophages and MIF mRNA+cells in H. pylori positive,compared with H.pylori negative patients,in both the antral and body mucosa.Moreover,the cell numbers increased with more severe chronic gastritis in both the antrum and body.The numbers were also significantly higher in ulcer patients than in NUD patients, particularly in H. pylori positive patients.In vitro,the expression of MIF protein and mRNA in monocytes was significantly increased by incubation with H.pylori whole cell proteins,in a time and dose dependent manner.CONCLUSIONS H.pylori infection stimulates the expression of MIF in the gastric inflammatory cells,which may play a significance role in gastric inflammation and ulceration.