Nocvel potential targets and related genes of transcription factor Caplp in Candida albicans 1

• 作者: De-junWANG HuiSHEN KangYING Wan-shengCHEN Xin-mingJIA YahWANG Yong-bingCAO Yuan-yingJIANG ZhengXU
• 来源期刊: 中国药理通讯
• 学科: 医学
• 关键词: 遗传基因 转录因子 Caplp 白色假丝酵母1 氧化作用
• 年，卷（期）: 2005,（2）
• 页码范围: 23
• 页数: 1页
• 分类号: R394

摘要

AIM Capl p, encoded by CAP1 in Candida albicans, is highly homologous to Saccharomyces cerevisiae transcription factor Yapl p. It has been associated with tolerance to oxidative stress and resistance to a variety of toxicants previously. We used homemade microarray to reveal Capl p related genes in a broad spectrum as well as to lucubrate the functions of Capl p. METHODS Microarray analysis was used to identify differentially expressed genes between CAP1 deletion strain CJD21 and its parental strain CAI4. CAP1 over-expression strain was constructed to confirm the relationship between CAP1 and some differentially expressedgenes. Bioinformatics was applied to reveal promoters with Capl p binding site as well as the clusters of differentially expressed genes. RT-PCR and drug efflux analysis were used to lucubrate the functions of Caplp in Candida albicans.