Muscle and adipose tissue biopsy in older adults with type 2 diabetes

• 作者: Anthony O’Sullivan Bernhard T. Baune David Simar Kylie Anderberg Maria Fiatarone Singh Nalin Singh Renru Zhao Shelley Kay Yi Wang Yorgi Mavros
• 来源期刊: 糖尿病(英文)
• 学科: 医学
• 关键词: BIOPSY SKELETAL MUSCLE SUBCUTANEOUS ADIPOSE TISSUE Type 2 Diabetes
• 年，卷（期）: 2011,（3）
• 页码范围: 27-35
• 页数: 9页
• 分类号: R5

摘要

We describe a method for obtaining skeletal muscle and subcutaneous adipose tissue in a single percutaneous needle biopsy procedure. Biopsies were performed in a double-blind randomized, sham-exercise controlled clinical trial of power training in 103 older adults with type 2 diabetes. In total 110 biopsies (73.3%) were performed in the first 50 participants at 3 time points. Muscle samples were obtained in 102/110 biopsies (92.7%);adipose samples were obtained in 72/110 biopsies (65.5%);both tissue types were obtained in 65/110 biopsies (59.1%). The average weight of muscle samples was 131±92 mg and the median weight of adipose samples was 51 mg (range 3 to 412 mg). No adverse effects of biopsies were reported other than mild transient pain or light-headedness. This is one of the first reports of both adipose and muscle tissue obtained during the same biopsy, and the first to describe in detail sampling technique, yield and safety.