Real Time Monitoring of Fluorescent Particles in Micro-Channels by High Resolution Dual Modality Probe Imaging

• 作者: Krishnan Sathiyamoorthy Vadakke Matham Murukeshan Valiyambath Krishnan Mohankumar
• 来源期刊: 光学与光子学期刊(英文)
• 学科: 医学
• 关键词: MICROSCOPY Image Fiber SPECTROPHOTOMETER MICROCHANNEL
• 年，卷（期）: 2011,（4）
• 页码范围: 197-203
• 页数: 7页
• 分类号: R73

摘要

Imaging of micro particles in micro-fluidic channels is one of the recent thrust areas in research as it provides more flexibility in setup and analysis compared to conventional microscopy. However, a probe based imaging scheme, with achievable high resolutions incorporating multimodal analysis is one of the challenges researchers have been facing. In this context, this paper illustrates a simple dual modality high resolution flexible probe imaging system for imaging applications in micro/optofluidic channels. The proposed system exhibits axial and lateral resolution of about 16 μm and 3.12 μm respectively. This proposed system also exhibits a modulation transfer function (MTF) of about 38.42 %. The performance of the system is validated by imaging micro particles in a microchannel and obtaining the fluorescent emission spectrum simultaneously.