Modulation of heat shock protein response in SH-SY5Y by mobile phone microwaves

• 作者: Daniela Caccamo Emanuele Calabrò Monica Currò Nadia Ferlazzo Riccardo Ientile Salvatore Condello Salvatore Magazù
• 来源期刊: 世界生物化学杂志：英文版（电子版）
• 学科: 生物学
• 关键词: Apoptosis ELECTROMAGNETIC field Heat shock PROTEINS Microwaves SH-SY5Y DIFFERENTIATED cells
• 年，卷（期）: 2012,（2）
• 页码范围: 34-40
• 页数: 7页
• 分类号: Q51

摘要

AIM: To investigate putative biological damage caused by GSM mobile phone frequencies by assessing electromagnetic fields during mobile phone working. METHODS: Neuron-like cells, obtained by retinoicacid-induced differentiation of human neuroblastoma SH-SY5Y cells, were exposed for 2 h and 4 h to microwaves at 1800 MHz frequency bands. RESULTS: Cell stress response was evaluated by MTT assay as well as changes in the heat shock protein expression (Hsp20, Hsp27 and Hsp70) and caspase-3 activity levels, as biomarkers of apoptotic pathway. Under our experimental conditions, neither cell viability nor Hsp27 expression nor caspase-3 activity was significantly changed. Interestingly, a significant decrease in Hsp20 expression was observed at both times of exposure, whereas Hsp70 levels were significantly increased only after 4 h exposure. CONCLUSION: The modulation of the expression of Hsps in neuronal cells can be an early response to radiofrequency microwaves.