Determination of Cholesterol Content in Waste Fat by Dual-wavelength Spectrophotometry

• 作者: LIU Jia-di QIN Jin-ping XU Chun-xiang XU Shu-jun
• 来源期刊: 中国食品科学：英文版
• 学科: 化学
• 关键词: 双波长分光光度法 分光光度法测定 胆固醇含量 脂肪 液相色谱法 废水 相对标准偏差 干扰隔离
• 年，卷（期）: 2012,（2）
• 页码范围: 40-42
• 页数: 3页
• 分类号: O657.32

摘要

[Objective]The aim was to find a way to measure the cholesterol content in deserted oil.[Method]Dual-wavelength spectrophotometry method was used for the determination of cholesterol in edible oil.630 nm and 720 nm were set as target band and reference band in this method,respectively.The result was compared to liquid chromatography method.[Result]The relative standard deviation(RSD)was at 1.26% and recovery was between 101.9% and 110.7%.Linear range was 8.0-40.0 μg/ml.The tested result was close to the result of liquid chromatography.[Conclusion]It was proved that this method was easy,fast and accurate.The disturbance of sitosterol was eliminated without isolation.It can be used for fast identification of the authenticity of waste fat.