Separation of Antitumor Active Plypeptides from Periplaneta Americana by Ultra Filtration Technology

• 作者: WU Shao-hui PENG Fang GAO Peng-fei WU Xiu-mei HE Miao ZHANG Cheng-gui LIU Guang-ming
• 来源期刊: 植物病虫害研究：英文版
• 学科: 农学
• 关键词: 植物病虫害 预防 植物保护 植物检疫
• 年，卷（期）: 2012,（1）
• 页码范围: 59-61
• 页数: 3页
• 分类号: S4

摘要

[Objective] The paper was to establish ultrafiltration membrane technology for separation of antitumor active plypeptides from Periplaneta Americana. [Method] Hollow fiber ultrafiltration membrane(3 kDa)was used to separate antitumor active plypeptides from P. Americana. Through single factor test, the impact of rotation speed, concentration ratio, pH and operation time on separation effort was studied, and the separation technology conditions were further optimized through orthogonal test. [Result] The best technology conditions for separation of antitumor active plypeptides from P. Americana were as follows: rotation speed 4 500 r/min, concentration ratio 30%, pH 11, and the rotation speed was the major influencing factor. [Conclusion] The study laid foundation for industrialization of ultrafiltration membrane separation of antitumor active plypeptides from P. Americana.