Human Embryo Neuronal Culture <i>in Vitro</i>: A Model to Study Cellular Physiology, Receptors, Power and Toxicity of Cytostatic Drugs for Human Use

• 作者: Altieri Vincenzo Buoninfante Luca Della Ragione Carlo Iuorio Tina Marino Lucia Masillo Francesco Monaco Roberto Stabile Mariano
• 来源期刊: 神经系统科学与医药(英文)
• 学科: 医学
• 关键词: HUMAN Embryo Neuronal Culture ERα on Embryonic Brain Mitomycin C TOXICITY TEST in VITRO Methotrexate TOXICITY TEST in VITRO
• 年，卷（期）: 2012,（3）
• 页码范围: 321-326
• 页数: 6页
• 分类号: R73

摘要

Neural cells cultures from human embryo brain of 9° - 11°W gestational age have been used to study ERα (Estrogens Receptor α) and to perform toxicity test for Mitomycin C and Methotrexate. Histochemical confirmation of cellular neuronal phenotype was based on histochemical evidence of NSE (Neuron Specific Enolase).The detection of ERα in neuronal cells was performed with a rabbit Monoclonal Antibody. ERα was absent both on neurons grown in vitro and on tissue brain specimens. This finding is apparently in contrast with the positive immunoreactivity of ERα and ERβ reported by other Authors on foetal and adult CNS (Central Nervous System). The absence of nuclear ERα on neurons in culture and in brain tissue specimens in our experiment is not in contrast with the relevant physiologic role of estrogens on nervous central system, but it could be correlated to the embryonic period of life and could represent a protection of male brain from an undue estrogens imprinting. The mitomycin C, alkylation agent, has shown in our experiment a major neurotoxic and cytostatic power in comparison with methotrexate. Our conclusion is that human embryo neuronal culture in vitro is a powerful instrument for physiology and human therapy for cancer and neurodegenerative diseases.