The Research on Identification of Resistance to Sor- ghum Aphid [ Melanaphis Sacchari （ Zehntner ） ] Employing Indoor Artificial Infestation Technique

• 作者: Du Ruiheng Hou Shenglin Ji Guisu Li Suying Liu Guoqing Lv Peng Ma Xue Qi Yameng
• 来源期刊: Plant Diseases and Pests(植物病虫害研究：英文版)
• 学科: 农学
• 关键词: 苗期蚜虫 抗性鉴定 繁殖技术 高粱蚜 室内 人工接虫 接种试验 甘蔗
• 年，卷（期）: pdapzwbchyj_2013,（3）
• 页码范围: 26-32
• 页数: 7页
• 分类号: S562

摘要

[Objective] The paper was to establish a quick and effective indoor identification method of sorghum resistance to aphid uninfluenced by seasons.[Method] Based on the indoor reproduction technique of aphids,the sorghum varieties with different resistant performances against aphids in fields were conducted inoculation test in the laboratory according to different seedling culture media,different survey periods of aphid quantity,different leaf ages for inoculation,different inoculation quantity of aphids and different aphid sizes. Three hundred sorghum materials were identified in both laboratory and field. [Result] The number of aphids per seedling on the fifth day after inoculation gave the best score of plant resistance to sorghum aphid by using the loam as the seedling culture media,and inoculating three different sizes of aphids in seedlings at two-leaf stage. Thus,the indoor identification technique of resistance to sorghum aphids was as follows:(1) sorghum seeds were sown in loam at room temperature of 22-26 ℃ and air humidity of 55%-75%;(2) three aphids with different sizes were inoculated in seedlings at two-leaf stage;(3) the number of aphids per seeding were surveyed on the fifth day after inoculation;(4) plant resistance to sorghum aphid was evaluated according to number of aphids per seedling on the fifth day after inoculation. The resistant grade of sorghum variety was evaluated according to distribution of aphid quantity per seedling in a 100-seedlings population. The resistance materials identified by indoor method at seedling stage had consistent performance with field identification. [Conclusion] The method could be used for resistance identification of sorghum varieties and hybrids,and accurate identification and selection of individual resistance of hybrid offspring,which could improve selection efficiency of individual resistant plant against aphids in hybrid offspring and identification accuracy of individual resistant plant among molecular marker population.