在照耀的黑瘤房间的精力新陈代谢和信号 transduction 的 Proteomic 分析

• 作者: Kai Shi Lu-Bin Yan Yang Shen Yi-Lan Sun Zhi-Tong Bing
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 关键词: MELANOMA cell 2D-LC-MS/MS stable ISOTOPE LABELING with amino acids PROTEOMIC analysis X-ray irradiation PROTEIN-PROTEIN interaction
• 年，卷（期）: 2013,（3）
• 页码范围: 286-294
• 页数: 9页
• 分类号: R739.5

摘要

AIM: To analyze proteomic and signal transduction alterations in irradiated melanoma cells. ·METHODS: We combined stable isotope labeling with amino acids in cell culture (SILAC) with highly sensitive shotgun tandem mass spectrometry (MS) to create an efficient approach for protein quantification. Protein - protein interaction was used to analyze relationships among proteins. · RESULTS: Energy metabolism protein levels were significantly different in glycolysis and not significantly different in oxidative phosphorylation after irradiation. Conversely, tumor suppressor proteins related to cell growth and development were downregulated, and those related to cell death and cell cycle were upregulated in irradiated cells. · CONCLUSION: Our results indicate that irradiation induces differential expression of the 29 identified proteins closely related to cell survival, cell cycle arrest, and growth inhibition. The data may provide new insights into the pathogenesis of uveal melanoma and guide appropriate radiotherapy.