Horseradish Peroxidase Biosensor to Detect Zinc Ions in Aqueous Solutions

• 作者: Mambo Moyo
• 来源期刊: 生物传感器(英文)
• 学科: 化学
• 关键词: HORSERADISH PEROXIDASE Maize TASSEL Multiwalled Carbon Nanotubes Metal BIOSENSOR Zn2+
• 年，卷（期）: 2014,（1）
• 页码范围: 1-7
• 页数: 7页
• 分类号: O6

摘要

Maize tassel-multiwalled carbon nanotube (MT-MWCNT) composite has been used as a matrix for physical adsorption of horseradish peroxidase (HRP) onto the surface of a glassy carbon electrode through electrostatic interactions. The HRP/MT-MWCNT biosensor was applied for the detection of Zn2+ in aqueous solution. The biosensor designed was able to determine Zn2+ in the range of 0.35 - 12 mg/L with a detection limit of 7.5 μg/L. The inhibition was found to be reversible and uncompetitive when data were modeled using the Dixon and Cornish-Bowden plots. The biosensor was found to have good repeatability, reproducibility and high selectivity. The developed biosensor can be used to detect other HRP inhibiting trace metal ions.