Cloning and Sequencing Characteristics of rDNA ITS Region of the Pathogen Causing Black Spot Disease on Tumorous Stem Mustard

• 作者: Liu Hongfang
• 来源期刊: Plant Diseases and Pests(植物病虫害研究：英文版)
• 学科: 农学
• 关键词: RDNA序列 ITS区 茎瘤芥 黑点病 分析特性 克隆 病原菌 碱基序列
• 年，卷（期）: pdapzwbchyj_2014,（1）
• 页码范围: 17-20
• 页数: 4页
• 分类号: S435.621.2

摘要

Taking tumorous stem mustard infected by black spot disease as the research material,the ribosomal 5. 8S rDNA and its flanking ITS region were cloned,sequenced and aligned in the study. The results showed that the base sequence of pathogens collected from five different sites was almost identical with that of Alternaria brassicae,and the similarity degree reached 99. 68%,without base difference greater than 3 bp. It had obvious differences with the sequences of A.brassicicola and A. japonica,and the base difference was greater than 3 bp with a lot of fragment deletions. It was preliminarily determined that the pathogen causing black spot disease on tumorous stem mustard was A. brassicae.