Intramedullary Spinal Cord Glioma Following Microinjection of Glioblastoma Cell Line C6 in Rats

• 作者: Elcin Hakan Terzi Gulzade Ozyalvacli Kaan Yagmurlu Tulin Firat Yasar Dagistan
• 来源期刊: 现代神经外科学进展(英文)
• 学科: 医学
• 关键词: INTRAMEDULLARY Spinal Cord GLIOMA Animal Model C6 GLIOBLASTOMA Cell
• 年，卷（期）: 2014,（1）
• 页码范围: 43-46
• 页数: 4页
• 分类号: R73

摘要

Background: This paper describes the establishment of a rat intramedullary spinal cord tumor (IMSCT) model and histopathological characterization of the tumor model. Methods: Fourteen male Wistar rats were randomized into two groups. The rats in group 1 (control group, n = 7) received a 5 μl intramedullary injection of serum physiologic (SF). Those in group 2 (experimental group, n= 7) received a 5 μl intramedullary implantation of media containing 5 × 105 C6 glioma cells. The animals were sacrificed for histopathological examination at 21 days. Results: The control group showed normal functional and histopathological findings. The group 2 rats implanted with C6 glioblastoma cells developed hind-limb paraplegia. Pathological sections confirmed intramedullary C6 glioblastoma invading the spinal cord. Conclusions: A rat C6 IMSCT model was successfully established. This model may be useful in increasing understanding of intramedullary spinal cord gliomas in humans.