3-48 High-LET Radiation Induced Mitophagy and Mitochondrial Apoptosis in Breast Cancer Cells

• 作者: Jin Xiaodong Li Qiang Zheng Xiaogang
• 来源期刊: 中国科学院近代物理研究所和兰州重离子研究装置年报：英文版
• 学科: 物理学
• 关键词: RADIATION INDUCED MITOPHAGY
• 年，卷（期）: 2014,（1）
• 页码范围: 142-142
• 页数: 1页
• 分类号: O4

摘要

To elucidate mitochondrial damage induced by high-LET radiation, human breast cancer MDA-MB-231 andMCF-7 cells were irradiated with carbon ions of 75 keV/m at different doses. Mitochondrial ΔΨm was stainedby JC-1 and measured by means of flow cytometry at 1, 4 and 24 h after irradiation, respectively. We found thatΔΨm had no or a slight change when the radiation dose was as low as about 0.2 or 0.5 Gy at any time point whiledissipating at higher doses in MDA-MB-231 cells (Fig. 1). This suggests that the mitochondrial membrane potential had no significant change under low stress conditions.However, high levels of cell stress led to the serious mitochondrialdysfunction.