The cultivation experiment was carried out on five species of Gesneriaceae plants( Oreocharis benthamii var. reticulate,O. argyreia,O.benthamii,Chirita laxiflora,and C. depressa). The growth traits including crown volume growth,number of leaves and chlorophyll content of each plant cultivated in different pH substrates were measured and analyzed. The pH and conductivity of the original habitat soil were measured as well.The results showed that these five species of Gesneriaceae preferred acidic environment,which could grow well in the matrix of 5- 6 pH range with the steady physiological indicators. The physical and chemical properties of the original habitat soil showed that these five species were resistant to barren,while favored humid environment and a large quantity of water. It is necessary to adjust physical and chemical properties of matrix according to their habits to improve their growth in the cultivation.