Experimental Validation and Simulation of Fourier and Non-Fourier Heat Transfer Equation during Laser Nano-Phototherapy of Lung Cancer Cells: An <i>in Vitro</i>Assay

• 作者: Lida Ghazanfari Mohammad E. Khosroshahi Payam Khoshkenar
• 来源期刊: 现代物理(英文)
• 学科: 医学
• 关键词: Bioheat Equation Gold NANOSHELLS Laser HYPERTHERMIA Lung Cancer Cells Surface PLASMON Resonance
• 年，卷（期）: xdwlyw_2014,（18）
• 页码范围: 2125-2141
• 页数: 17页
• 分类号: R73

摘要

This paper investigated the numerical scheme extended to solve the hyperbolic non-Fourier form of bioheat transfer equation and the experimental trials were conducted to validate the numerical simulation. MNPs were prepared via co-precipitation and modified with a silica layer. The amino modified Fe3O4/SiO2 nanoshells were covered with gold colloids producing nanoshells of Fe3O4/SiO2/Au (MNSs). In vitro assays were performed to determine the effect of apoptosis of QU-DB lung cancer cells based on the cells morphology changes. Cell damage was reduced by decreasing the power density of laser. Also, a larger area of damage on cell culture plates was observed at longer intervals of laser irradiation. The effect of nanoshell concentration and irradiation rate has been evaluated. A maximum temperature rise of 6°C was achieved at 184 W/cm2 and concentration of 0.01 mg/ml. The experiment confirmed a hyperbolic behaviour of thermal propagation. The results revealed that the three-dimensional implementation of bioheat equation is likely to be more accurate than the two-dimensional study.