The independence number of a graph G is the maximum cardinality among all independent sets of G. For any tree T of order n ≥ 2, it is easy to see that . In addition, if there are duplicated leaves in a tree, then these duplicated leaves are all lying in every maximum independent set. In this paper, we will show that if T is a tree of order n ≥ 4 without duplicated leaves, then . Moreover, we constructively characterize the extremal trees T of order n ≥ 4, which are without duplicated leaves, achieving these upper bounds.